What Is Measured in a Male Semen Analysis?
Semen is the fluid released during ejaculation, containing sperm cells that fertilize the egg. In a standard semen analysis, a sample is collected and assessed for various parameters that impact fertility. Here’s what a semen test measures:

1. Semen Volume
This refers to the total amount of fluid ejaculated. Normal semen volume ranges between 2-5 milliliters (ml).
- Low volume (<1.5 ml) (Hypospermia) → Possible hormonal imbalances or blockages
- High volume (>5.5 ml) (Hyperspermia) → May indicate an underlying condition
2. Sperm Count (Sperm Concentration)
Sperm count measures how many million sperm cells are present per milliliter of semen.
- Normal: ≥15 million sperm per ml
- Low sperm count (Oligospermia): <15 million per ml
- No sperm present (Azoospermia): Complete absence of sperm

3. Sperm Motility (Movement Ability)
Sperm motility determines how well sperm swim towards the egg. It’s graded as:
- Progressive Motility (PR): Sperm move in a straight line or large circles
- Non-Progressive Motility (NP): Sperm show movement but without effective direction
- Immotility (IM): No movement at all
A healthy sample should have at least 32% progressively motile sperm or 40% when including non-progressive motility. Poor motility, known as Asthenozoospermia, can reduce the likelihood of conception.
4. Total Motile Sperm Count (TMSC)
TMSC represents the total number of moving sperm in the ejaculate, calculated by multiplying:
Semen volume (ml) × Sperm concentration (million/ml) × Motility (%)
A healthy TMSC should be above 45 million sperm cells to optimize fertility chances.
Additional Semen Parameters for a More Detailed Analysis
If abnormalities are detected, a more advanced semen analysis may be needed, evaluating:
5. Sperm Morphology (Size & Shape)
Sperm morphology assesses how many sperm have a normal oval shape, as per Kruger’s strict criteria:
Normal sample: At least 4% of sperm have a standard shape
Teratozoospermia: Less than 4% normal-shaped sperm
Misshapen sperm may struggle to move or penetrate the egg, impacting fertility.

6. Semen Color & Viscosity
- Normal: Grey-opalescent
- Red/Brown Tint: May indicate blood in semen (Haemospermia)
- Viscosity: Semen should liquefy within 20 minutes—delays may signal infection.

7. Semen pH Levels
Semen is slightly alkaline (pH 7.2-8.0). Abnormal pH levels can:
✔️ Kill sperm
✔️ Reduce motility
✔️ Lower fertilization chances
8. Sperm Vitality (Live vs. Dead Sperm)
Vitality measures the percentage of live sperm in a sample. A normal result is:
- ≥58% live sperm
- Necrozoospermia: Fewer than 58% viable sperm
9. White Blood Cells (Infection Indicator)
Elevated white blood cells (Leukospermia) can indicate infection or inflammation, which may reduce sperm quality.
10. Essential Nutrients in Semen
Certain substances in semen are crucial for sperm function:
- Zinc: ≥2.4 µmol/ejaculate
- Fructose: ≥13 µmol/ejaculate (provides energy for sperm)
- Glucosidase: ≥20 µU/ejaculate (linked to sperm maturation)
Why Is a Semen Analysis Important for Male Fertility?
- Fast & Reliable Fertility Insights
A semen analysis offers quick results that help pinpoint any fertility issues early.
- Helps Improve Sperm Health
If results are abnormal, lifestyle adjustments (diet, exercise, stress management) can boost sperm quality in just 72 days.
- Saves Time, Money & Emotional Strain
Instead of months (or years) of unsuccessful attempts, an early semen analysis helps couples make informed decisions sooner.
- Key Indicator of Overall Health
Poor semen quality can be a sign of hormonal imbalances, nutrient deficiencies, or even underlying medical conditions.